Staff Scientist, PhD, Chief of Spectroscopy Group.
Bogdan S. Melnik was born 27.07.1969, Uzhgorod, Ukraine, USSR.
1986 - 1997 - Undergraduate student in chair of Biophysics, Department of Physics, St.Petersburg State University.
1994 - Bachelor's degree in Physics.
1997 - Master's degree in Biophysics.
1997 - 2000 - Ph.D. student in the Institute of Protein Research, Russian Academy of Science.
2005 - PhD
He studied large oligomeric protein GroEL and developed software to analyzing small-angle X-ray scattering data to model the solution structure of GroEL protein.
The results show that the model solution structure of unliganded GroEL differs from the crystal one due to changes of the interring distance as well as the position of the protein subunits and domains.
He took part also in studying of fast folding/unfolding kinetics of apomyoglobin. Was proposed a method by using of that a complicated experimental chevron plot can be deconvolved into the rates of two separated transitions.
His theoretical work has concern in developing of a simple approach to identify domain boundaries in proteins of unknown three-dimensional structure and analyzing of a systematic difference in residue contacts between X-ray- and NMR-resolved protein structures.
Experimental and theoretical studies of a protein structures and folding.
Garbuzinskiy S.V., Melnik BS., Filkelstein A.V., Galzitskaya OV. "Is there a systematic difference in residue contacts between X-ray- and NMR-resolved protein structures?" Proteins. 2005. (in press)
Galzitskaya OV, Melnik BS.
Prediction of protein domain boundaries from sequence alone. Protein Sci. 2003 Apr;12(4):696-701. PubMed
B.S.Melnik, A.A.Timchenko, K.Kimura, H.Kihara, K.Kuwajima, G.V.Semisotnov. The ligand induced large-scale conformational changes of GroEL chaperonin in solution demonstrated by small-angle X-ray scattering. EURESCO Conference and FEBS Advanced Course Biology of molecular chaperones 2003 august 30- sept. 4 Tomar, Portugal.
Timchenko AA, Melnik BS, Kihara H, Kimura K, Semisotnov GV.
GroES co-chaperonin small-angle X-ray scattering study shows ring orifice increase in solution. FEBS Lett. 2000 Apr 14;471(2-3):211-4. PubMed
A.A.Timchenko, B.S.Melnik, H.Kihara, K.Kimura, G.V.Semisotnov. Conformational plasticity of GroEL/ES chaperonins studied by synchrotron X-Ray diffuse scattering. Photon Factory Workshop on: New prospects of non-crystalline structural science by X-Ray and neutron diffraction/scattering. KEK, Tsukuba, Japan, 15-20, December, 2000.
B.S.Melnik, A.A.Timchenko, N.V.Kotova, G.V.Semisotnov. The conformational changes in solution structure of the chaperonin GroEL/ES by small-angle X-ray scattering International Symposium Protein structure, stability and folding. Fundamental and medical Aspects. Moscow, Russia. June 22-26, 1998
G.V.Semisotnov, T.Ikura, K.Ichimura, H.Kihara, K.Kimura, N.V.Kotova, B.S.Melnik, A.A.Timchenko and K.Kuwajima. Urea-induced unfolding of the oligomeric proteins GroEL and GroES studied by small-angle X-ray scattering Photon Factory Activity Report 1996 #14
G.V.Semisotnov, A.A.Timchenko, H.Kihara, K.Kimura, N.V.Kotova, B.S.Melnik, Y.Igarashi, K.Ichimura, S.Matsuzaki and K.Kuwajima. The solution structure of the chaperonin GroEL/ES studied by small-angle X-ray scattering Photon Factory Activity Report 1996 #14
A.A.Timchenko, H.Kihara, N.V.Kotova, V.P.Kutyshenko, B.S.Melnik, A.K.Surin, G.V.Semisotnov. Conformation and stability of the co-chaperonin GroES. 2nd Workshop Principles of Protein Architecture Waseda University International Convention Center 1-6-1 Nishiwaseda, Shinjuku-ku, Tokyo December 12-13, 1995.
A.K.Surin, I.A.Kashparov, H.Kihara, N.V.Kotova, V.D.Marchenkov, S.Yu.Merchenkova, B.S.Melnik, A.A.Timchenko, G.V.Semisotnov. Nucleotide action on the stability and conformation of chaperonin GroEL/ES. 2nd Workshop Principles of Protein Architecture Waseda University International Convention Center 1-6-1 Nishiwaseda, Shinjuku-ku, Tokyo Dec. 12-13, 1995.