Experimentally found Φ-values (larger 0.5) and predicted amyloidogenic regions

Protein or domain name	Size	Number of residues with known Φ-values	Predicted amyloidogenic regions	Residues with experimental Φ-values larger than 0.50 (Φ-value)^a
Spliceosomal protein U1A	96	10	9–15; 30–45; 53–59	44(0.60)
Ribosomal protein S6	97	10	1–9; 45–51	6(0.52)
B-domain of staphylococcal protein A	60	15	14–21	14(0.65); 17(1.0); 18(1.0); 32(0.57)
Colicin E9 immunity protein	86	19	15–22; 34–40	13(0.98); 15(0.57); 16(0.52)
Barnase	110	19	87–98; 103–109	13(0.60); 15(0.80); 57(1.0); 87(0.80); 90(0.9); 91(1.0); 96(0.40)
Chymotrypsin inhibitor (CI2)	64	34	29–35; 39–52	18(0.70); 49(0.53)
Ig repeat 27 of titin	89	22	56–64	23(0.82); 49(0.66); 56(0.52); 58(0.79); 60(0.67); 71(0.63); 75(0.63)
IgG binding domain of streptococcal protein L	64	38	7–13	4(0.51); 7(0.62); 8(0.53); 14(0.85); 21(0.75)
Villin 14T domain 1	126	18	18–24; 42–49; 58–66	23(0.65); 28(0.58); 44(0.85); 46(0.69); 48(0.62); 73(0.69); 77(0.52); 78(0.56); 81(0.75); 84(0.68)
10th FN3 module of fibronectin	94	16	28–35; 69–75	8(0.55); 34(0.61); 36(0.52); 72(0.51); 74(0.65); 92(0.79)
DNA-binding protein Sso7d	64	15	22–26; 30–34^b	25(0.65); 45(0.59); 57(0.54); 59(0.60)
Barstar	89	22	35–56	5(0.63); 9(0.72); 18(0.69); 25(0.68); 36(0.70); 37(0.59); 50(0.77); 68(0.52); 72(0.81);
Human muscle acylphosphatase	98	17	19–25; 91–98	11(0.93); 47(0.54); 54(0.98); 94(0.76)
FK-506 binding protein (FKBP12)	107	22	96–107	23(0.52); 27(0.85); 63(0.51); 101(0.57)
SH3 domain of src tyrosine kinase transforming protein	64	26	9–16; 41–47	30(0.62); 32(0.55); 35(0.77); 44(0.54); 47(0.95); 48(0.72); 50(0.86); 53(0.68); 55(0.56); 56(0.71)
Colicin E7 immunity protein	87	19	34–44	3(0.52); 7(0.56); 16(0.72); 18(0.52); 27(0.56); 37(0.51); 44(1.0); 68(0.86); 77(0.86); 78(0.78)
CheY	128	9	16–22; 51–57; 80–86	35(0.75); 37(0.60); 41(0.68)
IgG binding domain of streptococcal protein G	56	20	1–7^b	32(0.55); 46(0.96); 47(0.67); 49(0.84)
SH3 domain of α-spectrin	62	13	8–14; 31–35; 40–44^b	47(0.63); 52(0.58); 53(0.61); 55(0.53)
3rd Fn3 repeat of tenascin (short form)	90	25	19–26	36(0.53); 48(0.67); 70(0.54)

^aIf several Φ-values are known for a single residue (several different mutations were made), the Φ-values were averaged.

^bFor these proteins, amyloidogenic regions are revealed only with window size of five residues. The size of the sliding window for the rest proteins was seven residues.

The fraction of all known experimental Φ-values in the predicted amyloidogenic regions is 25%, the fraction of large Φ-values (>0.5) within amyloidogenic fragments is 33%, such Φ-values are shown in bold in Table.

Summary graph

Experimentally measured and theoretically predicted Φ-values.